4T1-CAS9 cell line

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the 4T1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a 4T1-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
4T1-CAS9	Cas9	17.84605217	4.97329903
4T1-CAS9	β-actin	12.87275314	4.97329903
4T1	Cas9	32.99827957	20.31225967
4T1	β-actin	12.68601990	20.31225967

Cutting Efficiency Validation

4T1-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the 4T1-CAS9 stable cell pool which is electroporated by Atg5 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:4T1				STR Info (Cell bank) Cell Line:4T1
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
18-3	18.0	19.0			18.0	19.0
4-2	21.3				21.3
6-7	12.0				12.0
19-2	13.0				13.0
1-2	17.0				17.0
7-1	25.2				25.2
8-1	13.0				13.0
1-1	15.0	16.0			15.0	16.0
3-2	14.0	15.0			14.0	15.0
2-1	16.0	17.0			16.0	17.0
15-3	22.3				22.3
6-4	18.0				18.0
13-1	16.2				16.2
11-2	18.0	20.0			18.0	19.0	20.0
17-2	15.0				15.0
12-1	16.0				16.0
5-5	14.0				14.0
X-1	25.0				25.0
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

4T1-CAS9

Catalog#

YC-B004-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

KO Cell Lines

Atg5 Knockout cell line (4T1)
inquiry More details

Organism: Mouse

Gene Name: Atg5

Gene ID: 11793

Size: 1*10^6

Catalog#: YKO-M225

YKO-M225(4T1)
Pigr Knockout cell line (4T1)
inquiry More details

Organism: Mouse

Gene Name: Pigr

Gene ID: 18703

Size: 1*10^6

Catalog#: YKO-M224

YKO-M224(4T1)
Nlgn4l Knockout cell line (4T1)
inquiry More details

Organism: Mouse

Gene Name: Nlgn4l

Gene ID: 100113365

Size: 1*10^6

Catalog#: YKO-M191

YKO-M191(4T1)

Related Popular Products

KO Cell Lines

Atg5 Knockout cell line (4T1)

Organism: Mouse

Gene Name: Atg5

Gene ID: 11793

Size: 1*10^6

Catalog#: YKO-M225

inquiry More details
Pigr Knockout cell line (4T1)

Organism: Mouse

Gene Name: Pigr

Gene ID: 18703

Size: 1*10^6

Catalog#: YKO-M224

inquiry More details
Nlgn4l Knockout cell line (4T1)

Organism: Mouse

Gene Name: Nlgn4l

Gene ID: 100113365

Size: 1*10^6

Catalog#: YKO-M191

inquiry More details

4T1-CAS9 cell line

Subscribe Us

4T1-CAS9 Order

Detailed Product Information

Product Advantage

Related Popular Products

KO Cell Lines

Atg5 Knockout cell line (4T1) inquiry More details

Pigr Knockout cell line (4T1) inquiry More details

Nlgn4l Knockout cell line (4T1) inquiry More details

Related Popular Products

KO Cell Lines

Atg5 Knockout cell line (4T1)

Pigr Knockout cell line (4T1)

Nlgn4l Knockout cell line (4T1)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Contact us

Atg5 Knockout cell line (4T1)
inquiry More details

Pigr Knockout cell line (4T1)
inquiry More details

Nlgn4l Knockout cell line (4T1)
inquiry More details